Effect of MGO on inflammation in 3T3-L1 cells
AbstractAdvanced Glycation Endproducts (AGEs) play a major role in inducing adipose tissue inflammation associated with obesity-associated insulin resistance. MGO, a precursor for AGEs is accumulated during obesity. Therefore we hypothesized that increased MGO-derived AGEs induce inflammation in adipose tissue via the interaction with RAGE. Incubation of the 3T3-L1 adipocytes with MGO-modified albumin increased the inflammatory gene expression. In addition, RAGE gene expression was increased and GLO-1 gene expression was decreased. So, inflammation is induced in 3T3-L1 adipocytes by MGO-derived AGEs after upregulation of RAGE gene expression. RAGE-mediated MGO-induced inflammation is an important mechanism involved in the obesity-induced adipose tissue inflammation.
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