Galactooligosaccharides and substrate and energy metabolism, dietary intake and body composition


  • M.A.J. Dijck, van



Aim: Obesity is associated with an altered composition of the gut microbiota. Prebiotics such as galactooligosaccharides (GOS) might modulate the gut microbiota composition beneficially and might have favorable metabolic effects in overweight and obese subjects. The aim of the study is to determine the effect of GOS on human substrate and energy metabolism, dietary intake and body composition in overweight and obese subjects. Methods: In a placebo-controlled, double-blind, randomized, parallel study, the effects of GOS were investigated in overweight and obese subjects. Weight-stable men and post-menopausal women between 40 and 75 years were included. Before and after the 12-week intervention period human substrate and energy expenditure were measured by indirect calorimetry. Subjects reported their dietary intake before, in week 6 and after the intervention period by completing a three-day weighed food diary. Self-reported level of physical activity was assessed using the validated SQUASH questionnaire. Changes in body composition were measured using a Dual-energy X-ray absorptiometry (DEXA) scan. A two-factor mixed analysis of variance (ANOVA) was performed to compare the intervention and placebo group. All analyses were performed blinded. Results: This interim analysis was performed after the data of 25 subjects was available. The data was blinded while analyzing. To maintain confidentiality of the data, the results of this interim analysis were not published. Conclusion: Only a part of the whole subject population was included in this interim analysis. The analyses in the total study group are required to draw more definite conclusions on the metabolic effects of GOS and to outline the potential of GOS to favorably affect host metabolism of overweight and obese subjects.


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